EF-hand protein, EfhP, specifically binds Ca²⁺ and mediates Ca²⁺ regulation of virulence in a human pathogen Pseudomonas aeruginosa.

ABSTRACT: Calcium (Ca²⁺) is well known as a second messenger in eukaryotes, where Ca²⁺ signaling controls life-sustaining cellular processes. Although bacteria produce the components required for Ca²⁺ signaling, little is known about the mechanisms of bacterial Ca²⁺ signaling. Previously, we have identified a putative Ca²⁺-binding protein EfhP (PA4107) with two canonical EF-hand motifs and reported that EfhP mediates Ca²⁺ regulation of virulence factors production and infectivity in Pseudomonas aeruginosa, a human pathogen causing life-threatening infections. Here, we show that EfhP selectively binds Ca²⁺ with 13.7 µM affinity, and that mutations at the +X and -Z positions within each or both EF-hand motifs abolished Ca

SUBMITTER: Kayastha BB 

PROVIDER: S-EPMC9132961 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature