Long-term observation after transplantation of cultured human corneal endothelial cells for corneal endothelial dysfunction.

ABSTRACT:

Background

Corneal transplantation is the only way to treat serious corneal diseases caused by corneal endothelial dysfunction. However, the shortage of donor corneal tissues and human corneal endothelial cells (HCECs) remains a worldwide challenge. We cultivated HCECs by the use of a conditioned medium from orbital adipose-derived stem cells (OASC-CM) in vitro. Then the HCECs were used to treat animal corneal endothelial dysfunction models via cell transplantation. The purpose of this study was to conduct a long-term observation and evaluation after cell transplantation.

Methods

Orbital adipose-derived stem cells (OASCs) were isolated to prepare the conditioned medium (CM). HCECs were cultivated and expanded by the usage of the CM (CM-HCECs). Then, related corneal endotheli

SUBMITTER: Sun P 

PROVIDER: S-EPMC9166479 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature