Apigenin impedes cell cycle progression at G₂ phase in prostate cancer cells.

ABSTRACT: As a natural flavone, apigenin is abundantly present in vegetables, fruits, oregano, tea, chamomile, wheat sprout and is regarded as a major component of the Mediterranean diet. Apigenin is known to inhibit proliferation in different cancer cell lines by inducing G₂/M arrest, but it is unclear whether this action is predominantly imposed on G₂ or M phases. In this study, we demonstrate that apigenin arrests prostate cancer cells at G₂ phase by flow cytometric analysis of prostate cancer cells co-stained for phospho-Histone H3 and DNA. Concurrently, apigenin also reduces the mRNA and protein levels of the key regulators that govern G₂-M transition. Further analysis using chromatin immunoprecipitation (ChIP) confirmed the diminished transcriptional

SUBMITTER: Hnit SST 

PROVIDER: S-EPMC9174405 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature