Brain aging is faithfully modelled in organotypic brain slices and accelerated by prions.

ABSTRACT: Mammalian models are essential for brain aging research. However, the long lifespan and poor amenability to genetic and pharmacological perturbations have hindered the use of mammals for dissecting aging-regulatory molecular networks and discovering new anti-aging interventions. To circumvent these limitations, we developed an ex vivo model system that faithfully mimics the aging process of the mammalian brain using cultured mouse brain slices. Genome-wide gene expression analyses showed that cultured brain slices spontaneously upregulated senescence-associated genes over time and reproduced many of the transcriptional characteristics of aged brains. Treatment with rapamycin, a classical anti-aging compound, largely abolished the time-dependent transcriptional changes in naturally aged bra

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9177860 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature