Specular Microscopy of Human Corneas Stored in an Active Storage Machine.

ABSTRACT:

Purpose

Unlike corneas stored in cold storage (CS) which remain transparent and thin, corneas stored in organoculture (OC) cannot be assessed by specular microscopy (SM), because edema and posterior folds occur during storage and prevent from specular reflection. We previously developed an active storage machine (ASM) which restores the intraocular pressure while renewing the storage medium, thus preventing major stromal edema. Its transparent windows allow multimodal corneal imaging in a closed system.

Aim

to present SM of corneas stored in this ASM.

Methods

Ancillary study of two preclinical studies on corneas stored for one and three months in the ASM. A prototype non-contact SM was developed (CMOS camera, ×10 objective, collimated LED source, micrometric stage). F

SUBMITTER: Garcin T 

PROVIDER: S-EPMC9181187 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature