ANGT_HUMAN[448-462], an Anorexigenic Peptide Identified Using Plasma Peptidomics.

ABSTRACT: The discovery of bioactive peptides is an important research target that enables the elucidation of the pathophysiology of human diseases and provides seeds for drug discovery. Using a large number of native peptides previously identified using plasma peptidomics technology, we sequentially synthesized selected sequences and subjected them to functional screening using human cultured cells. A 15-amino-acid residue proangiotensinogen-derived peptide, designated ANGT_HUMAN[448-462], elicited cellular responses and bound to cultured human cells. Synthetic fluorescent-labeled and biotinylated ANGT_HUMAN[448-462] peptides were rendered to bind to cell- and tissue-derived proteins and peptide-cell protein complexes were retrieved and analyzed using liquid chromatography-tandem mass spectrometry,

SUBMITTER: Sasaki S 

PROVIDER: S-EPMC9184509 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature