An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies.

ABSTRACT: Conventional in vitro methods for biological evaluation of intra-arterial devices such as stents fail to accurately predict cytotoxicity and remodeling events. An ex vivo flow-tunable vascular bioreactor system (VesselBRx), comprising intra- and extra-luminal monitoring capabilities, addresses these limitations. VesselBRx mimics the in vivo physiological, hyperplastic, and cytocompatibility events of absorbable magnesium (Mg)-based stents in ex vivo stent-treated porcine and human coronary arteries, with in-situ and real-time monitoring of local stent degradation effects. Unlike conventional, static cell culture, the VesselBRx perfusion system eliminates unphysiologically high intracellular Mg²⁺ concentrations and localized O₂ consumption resulting from stent degradat

SUBMITTER: Wang J 

PROVIDER: S-EPMC9195126 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature