SARS-CoV-2 productively infects human brain microvascular endothelial cells.

ABSTRACT:

Background

The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB).

Methods

RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes.

Results

A total of 927 differentially expressed genes were identifi

SUBMITTER: Yang RC 

PROVIDER: S-EPMC9198209 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature