An Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Molecule Reveals Zn²⁺ Micro-Dynamics During Drug-Induced Organelle Ionic Disorder.

ABSTRACT: The endoplasmic reticulum (ER) is the main storage site of Zn²⁺, and Zn²⁺ plays an important role in regulating ER homeostasis. Therefore, we designed and synthesized a ratiometric fluorescent Zn²⁺ probe ER-Zn targeting ER stress. The probe displayed a specific Zn²⁺ induced blue shift at the spectral maximum values of excitation (80 nm) and emission (30 nm). The ratio imaging capability of Zn²⁺ under dual excitation mode can be applied not only to quantitative and reversible detection of exogenous Zn²⁺, but also the observation of the Zn²⁺ level change under ER stress, elucidating the different behaviors of Zn²⁺ release in ER stimulated by tunicamycin and thapsigargin. Additionally, the NIR imaging capa

SUBMITTER: Fang H 

PROVIDER: S-EPMC9207263 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature