CUT&RUN for Chromatin Profiling in Caenorhabditis elegans.

ABSTRACT: Cleavage under targets and release using nuclease (CUT&RUN) is a recently developed chromatin profiling technique that uses a targeted micrococcal nuclease cleavage strategy to obtain high-resolution binding profiles of protein factors or to map histones with specific post-translational modifications. Due to its high sensitivity, CUT&RUN allows quality binding profiles to be obtained with only a fraction of the starting material and sequencing depth typically required for other chromatin profiling techniques such as chromatin immunoprecipitation. Although CUT&RUN has been widely adopted in multiple model systems, it has rarely been utilized in Caenorhabditis elegans, a model system of great importance to genomic research. Cell dissociation techniques, which are required for this approach,

SUBMITTER: Emerson FJ 

PROVIDER: S-EPMC9219583 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature