Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma.

ABSTRACT: Exosomes that are released by T cells are key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires their isolation from a very heterogeneous mixture of extracellular vesicles that are present in the human plasma. It has been shown that exosomes that are produced by T cells could be isolated from plasma by immune capture using antibodies that target the CD3 antigen, which is a key component of the TCR complex that is present in all T lymphocytes. Here, we demonstrate that CD3(+) exosomes that are isolated from plasma can be used for high-throughput molecular profiling using proteomics and metabolomics tools. This profiling allowed for the identification of proteins and metabolites that di

SUBMITTER: Zebrowska A 

PROVIDER: S-EPMC9222142 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature