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Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells.

ABSTRACT: A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9233151 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells.

Zhang Yibin Y   Arachchige Dilka Liyana DL   Olowolagba Adenike A   Luck Rudy L RL   Liu Haiying H  

Methods (San Diego, Calif.) 20220402

A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction. ...[more]

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