A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination.

ABSTRACT: Eukaryotic cells use G-protein coupled receptors to sense diverse signals, ranging from chemical compounds to light. Here, we exploit the remarkable sensing capacity of G-protein coupled receptors to construct yeast-based biosensors for real-life applications. To establish proof-of-concept, we focus on cannabinoids because of their neuromodulatory and immunomodulatory activities. We construct a CB₂ receptor-based biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three applications of increasing difficulty. First, we screen a compound library to discover agonists and antagonists. Second, we analyze 54 plants to discover a new phytocannabinoid, dugesialactone. Finally, we develop a robust portable device, analyze body-fluid sample

SUBMITTER: Miettinen K 

PROVIDER: S-EPMC9237071 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature