Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis.

ABSTRACT:

Background

We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis.

Methods

Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Individuals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021.

Results

Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; P-value≤0.05). No cross-reactions were observ

SUBMITTER: Alizadeh G 

PROVIDER: S-EPMC9276597 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature