Investigation of Toxoplasma infection in zoo animals using multispecies ELISA and GRA7 nested PCR.

ABSTRACT:

Background

Toxoplasma is an obligate intracellular protozoan that causes an important zoonotic disease with a worldwide distribution. Felids are the definitive hosts of this parasite, while virtually all warm-blooded animals, including birds, serve as intermediate hosts. Four ring-tailed lemurs (Lemur catta) in the Taipei Zoo died of acute Toxoplasma infection in June 2019. Since then, Toxoplasma has occasionally been identified in this Zoo during necropsy of dead animals and PCR of animal blood samples. Therefore, a general survey of Toxoplasma infection in animals in the Zoo seems to be needed.

Methods and results

An indirect multispecies ELISA was used for the first time to screen for Toxoplasma infection in 326 serum samples collected from 75 species of animals. The infe

SUBMITTER: Liu RM 

PROVIDER: S-EPMC9447357 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature