RNA m¹A methylation regulates glycolysis of cancer cells through modulating ATP5D.

ABSTRACT: Studies on biological functions of RNA modifications such as N⁶-methyladenosine (m⁶A) in mRNA have sprung up in recent years, while the roles of N¹-methyladenosine (m¹A) in cancer progression remain largely unknown. We find m¹A demethylase ALKBH3 can regulate the glycolysis of cancer cells via a demethylation activity dependent manner. Specifically, sequencing and functional studies confirm that ATP5D, one of the most important subunit of adenosine 5'-triphosphate synthase, is involved in m¹A demethylase ALKBH3-regulated glycolysis of cancer cells. The m¹A modified A71 at the exon 1 of ATP5D negatively regulates its translation elongation via increasing the binding with YTHDF1/eRF1 complex, which fac

SUBMITTER: Wu Y 

PROVIDER: S-EPMC9282374 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature