Protocol for qPCR analysis that corrects for cDNA amplification efficiency.
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ABSTRACT:
SUBMITTER: Damgaard MV
PROVIDER: S-EPMC9283931 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
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Protocol for qPCR analysis that corrects for cDNA amplification efficiency.
Damgaard Mads V MV Treebak Jonas T JT
STAR protocols 20220711 3
This protocol presents a variation on the 2<sup>-ΔΔCt</sup> technique for qPCR analysis. Our approach requires the inclusion of a standard curve on each qPCR plate, and like the 2<sup>-ΔΔCt</sup> technique, is dependent on the stability of housekeeping gene expression. However, unlike the 2<sup>-ΔΔCt</sup> technique, our approach corrects for imperfect cDNA amplification efficiency and allows for the use of multiple housekeeping genes. Collectively, this approach enhances analytical accuracy and ...[more]
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