Comprehensive analysis of miRNA-mRNA interactions in ovaries of aged mice.

ABSTRACT: Advanced maternal age and ovarian aging are deleterious to the quantity and quality of oocytes and epigenetic modifications, which can affect the health of offspring. However, relatively little is known about the regulation of microRNA-mediated transcription during ovarian aging. We therefore aimed to identify age-related mRNA and microRNA changes and their interactions in the ovaries of aged mice. We performed QuantSeq 3'mRNA and small RNA sequencing to compare their expression patterns in post-ovulation ovaries from young (12-week-old) and old (44-week-old) mice. Functional annotation and integrative analyses were performed to identify the potential functions of differentially expressed genes and identify binding sites for critical microRNAs. We found 343 differentially expressed genes a

SUBMITTER: Kim J 

PROVIDER: S-EPMC9285582 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature