Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells.

ABSTRACT: Transplantation of pancreatic islet cells derived from human pluripotent stem cells is a promising treatment for diabetes. Despite progress in the generation of stem-cell-derived islets (SC-islets), no detailed characterization of their functional properties has been conducted. Here, we generated functionally mature SC-islets using an optimized protocol and benchmarked them comprehensively against primary adult islets. Biphasic glucose-stimulated insulin secretion developed during in vitro maturation, associated with cytoarchitectural reorganization and the increasing presence of alpha cells. Electrophysiology, signaling and exocytosis of SC-islets were similar to those of adult islets. Glucose-responsive insulin secretion was achieved despite differences in glycolytic and mitochondrial gl

SUBMITTER: Balboa D 

PROVIDER: S-EPMC9287162 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature