Dichotomous role of Shp2 for naive and primed pluripotency maintenance in embryonic stem cells.

ABSTRACT:

Background

The requirement of the Mek1 inhibitor (iMek1) during naïve pluripotency maintenance results from the activation of the Mek1-Erk1/2 (Mek/Erk) signaling pathway upon leukemia inhibitory factor (LIF) stimulation.

Methods

Through a meta-analysis of previous genome-wide screening for negative regulators of naïve pluripotency, Ptpn11 (encoding the Shp2 protein, which serves both as a tyrosine phosphatase and putative adapter), was predicted as one of the key factors for the negative modulation of naïve pluripotency through LIF-dependent Jak/Stat3 signaling. Using an isogenic pair of naïve and primed mouse embryonic stem cells (mESCs), we demonstrated the differential role of Shp2 in naïve and primed pluripotency.

Results

Loss of Shp2 increased naïve pluripotency

SUBMITTER: Kim SM 

PROVIDER: S-EPMC9290224 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature