Glucocorticoids promote CCL20 expression in keratinocytes.

ABSTRACT:

Background

Glucocorticoids (GCs) are generally envisioned as immunosuppressive, but in conditions such as rosacea and perioral dermatitis they can lead to increased skin inflammation. In lung epithelia, GCs promote expression of the proinflammatory cytokine CCL20, which contributes to steroid-resistant asthma. In the skin, CCL20 stimulates inflammation by recruiting T helper 17 T lymphocytes and dendritic cells, and is elevated in papulopustular rosacea.

Objectives

To understand if, and how, GCs affect CCL20 expression in human keratinocytes. CCL20 expression was assessed by quantitative reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay.

Methods

Selective inhibition of candidate genes and signalling pathways was performed using RNA

SUBMITTER: Wang L 

PROVIDER: S-EPMC9290737 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature