A novel transposable element-mediated mechanism causes antiviral resistance in Drosophila through truncating the Veneno protein.

ABSTRACT: Hosts are continually selected to evolve new defenses against an ever-changing array of pathogens. To understand this process, we examined the genetic basis of resistance to the Drosophila A virus in Drosophila melanogaster. In a natural population, we identified a polymorphic transposable element (TE) insertion that was associated with an ∼19,000-fold reduction in viral titers, allowing flies to largely escape the harmful effects of infection by this virulent pathogen. The insertion occurs in the protein-coding sequence of the gene Veneno, which encodes a Tudor domain protein. By mutating Veneno with CRISPR-Cas9 in flies and expressing it in cultured cells, we show that the ancestral allele of the gene has no effect on viral replication. Instead, the TE inserti

SUBMITTER: Brosh O 

PROVIDER: S-EPMC9304006 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature