Stable isotope dilution mass spectrometry quantification of hydrogen sulfide and thiols in biological matrices.

ABSTRACT:

Background

Hydrogen sulfide (H₂S), a gaseous signaling molecule that impacts multiple physiological processes including aging, is produced via select mammalian enzymes and enteric sulfur-reducing bacteria. H₂S research is limited by the lack of an accurate internal standard-containing assay for its quantitation in biological matrices.

Methods

After synthesizing [³⁴S]H₂S and developing sample preparation protocols that avoid sulfide contamination with the addition of thiol-containing standards or reducing reagents, we developed a stable isotope-dilution high performance liquid chromatography tandem-mass spectrometry (LC-MS/MS) method for the simultaneous quantification of Total H₂S and other abundant thiols (cysteine, hom

SUBMITTER: Malaeb H 

PROVIDER: S-EPMC9307673 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature