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Recent Advances in Improving Gene-Editing Specificity through CRISPR-Cas9 Nuclease Engineering.

ABSTRACT: CRISPR-Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR-Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.

SUBMITTER: Huang X

PROVIDER: S-EPMC9319960 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Recent Advances in Improving Gene-Editing Specificity through CRISPR-Cas9 Nuclease Engineering.

Huang Xiaoqiang X Yang Dongshan D Zhang Jifeng J Xu Jie J Chen Y Eugene YE

Cells 20220713 14

CRISPR-Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR-Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing spec ...[more]

PMID: 35883629

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