Continuous countercurrent chromatographic twin-column purification of oligonucleotides: The role of the displacement effect.

ABSTRACT: Oligonucleotides (ONs) are breaking through in the biopharmaceutical industry as a promising class of biotherapeutics. The main success of these molecules is due to their peculiar way of acting in the cellular process, regulating the gene expression and hence influencing the protein synthesis at a pretranslational level. Although the Food and Drug Administration (FDA) already approved a few ON-based therapeutics, their production cost strongly limits large-scale manufacturing: a situation that can be alleviated through process intensification. In this study, we address this problem by developing an efficient and continuous chromatographic purification process for ONs. In particular, we considered the chromatographic purification of an ON crude prepared by chemical synthesis using anion exc

SUBMITTER: Fioretti I 

PROVIDER: S-EPMC9322279 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature