Fast neutron mutagenesis in soybean enriches for small indels and creates frameshift mutations.

ABSTRACT: The mutagenic effects of ionizing radiation have been used for decades to create novel variants in experimental populations. Fast neutron (FN) bombardment as a mutagen has been especially widespread in plants, with extensive reports describing the induction of large structural variants, i.e., deletions, insertions, inversions, and translocations. However, the full spectrum of FN-induced mutations is poorly understood. We contrast small insertions and deletions (indels) observed in 27 soybean lines subject to FN irradiation with the standing indels identified in 107 diverse soybean lines. We use the same populations to contrast the nature and context (bases flanking a nucleotide change) of single-nucleotide variants. The accumulation of new single-nucleotide changes in FN lines is marginall

SUBMITTER: Wyant SR 

PROVIDER: S-EPMC9335934 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature