Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.

ABSTRACT:

Background

The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks.

Methods

In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min.

Results

The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 10³ RNA copies/μL at a 95% confidence interval according to probit regression analysis, with 100% specificity. Compared with published reverse t

SUBMITTER: Wang S 

PROVIDER: S-EPMC9338541 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature