Repurposing of a human antibody-based microarray to explore conserved components of the signalome of the parasitic nematode Haemonchus contortus.

ABSTRACT:

Background

Gaining insight into molecular signalling pathways of socioeconomically important parasitic nematodes has implications for understanding their molecular biology and for developing novel anthelmintic interventions.

Methods

Here, we evaluated the use of a human antibody-based microarray to explore conserved elements of the signalome in the barber's pole worm Haemonchus contortus. To do this, we prepared extracts from mixed-sex (female and male) adult worms and third-stage larvae (L3s), incubated these extracts on the antibody microarray and then measured the amounts of antibody-bound proteins ('signal intensity').

Results

In total, 878 signals were classified into two distinct categories: signals that were higher for adults than for larvae of H. contortus (n 

SUBMITTER: Adderley J 

PROVIDER: S-EPMC9338626 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature