Umbilical cord blood DNA methylation in children who later develop type 1 diabetes.
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ABSTRACT: Aims/hypothesis
Distinct DNA methylation patterns have recently been observed to precede type 1 diabetes in whole blood collected from young children. Our aim was to determine whether perinatal DNA methylation is associated with later progression to type 1 diabetes.Methods
Reduced representation bisulphite sequencing (RRBS) analysis was performed on umbilical cord blood samples collected within the Finnish Type 1 Diabetes Prediction and Prevention (DIPP) Study. Children later diagnosed with type 1 diabetes and/or who tested positive for multiple islet autoantibodies (n = 43) were compared with control individuals (n = 79) who remained autoantibody-negative throughout the DIPP follow-up until 15 years of age. Potential confounding factors related to the pregnancy and the mother were included in the analysis.Results
No differences in the umbilical cord blood methylation patterns were observed between the cases and controls at a false discovery rate <0.05.Conclusions/interpretation
Based on our results, differences between children who progress to type 1 diabetes and those who remain healthy throughout childhood are not yet present in the perinatal DNA methylome. However, we cannot exclude the possibility that such differences would be found in a larger dataset.
SUBMITTER: Laajala E
PROVIDER: S-EPMC9345803 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Publications
Umbilical cord blood DNA methylation in children who later develop type 1 diabetes.
Laajala Essi E Kalim Ubaid Ullah UU Grönroos Toni T Rasool Omid O Halla-Aho Viivi V Konki Mikko M Kattelus Roosa R Mykkänen Juha J Nurmio Mirja M Vähä-Mäkilä Mari M Kallionpää Henna H Lietzén Niina N Ghimire Bishwa R BR Laiho Asta A Hyöty Heikki H Elo Laura L LL Ilonen Jorma J Knip Mikael M Lund Riikka J RJ Orešič Matej M Veijola Riitta R Lähdesmäki Harri H Toppari Jorma J Lahesmaa Riitta R
Diabetologia 20220618 9
<h4>Aims/hypothesis</h4>Distinct DNA methylation patterns have recently been observed to precede type 1 diabetes in whole blood collected from young children. Our aim was to determine whether perinatal DNA methylation is associated with later progression to type 1 diabetes.<h4>Methods</h4>Reduced representation bisulphite sequencing (RRBS) analysis was performed on umbilical cord blood samples collected within the Finnish Type 1 Diabetes Prediction and Prevention (DIPP) Study. Children later dia ...[more]
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