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Multiplex base editing to convert TAG into TAA codons in the human genome.

ABSTRACT: Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and highly multiplex editing in mammalian cells.

SUBMITTER: Chen Y

PROVIDER: S-EPMC9345975 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Multiplex base editing to convert TAG into TAA codons in the human genome.

Chen Yuting Y Hysolli Eriona E Chen Anlu A Casper Stephen S Liu Songlei S Yang Kevin K Liu Chenli C Church George G

Nature communications 20220802 1

Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, ...[more]

PMID: 35918324

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