Identification of novel functional mini-receptors by combinatorial screening of split-WW domains.

ABSTRACT: β-Sheet motifs such as the WW domain are increasingly being explored as building blocks for synthetic biological applications. Since the sequence-structure relationships of β-sheet motifs are generally complex compared to the well-studied α-helical coiled coil (CC), other approaches such as combinatorial screening should be included to vary the function of the peptide. In this study, we present a combinatorial approach to identify novel functional mini-proteins based on the WW-domain scaffold, which takes advantage of the successful reconstitution of the fragmented WW domain of hPin1 (hPin1_WW) by CC association. Fragmentation of hPin1_WW was performed in both loop 1 (CC-hPin1_WW-L1) and loop 2 (CC-hPin1_WW-L2), and the respective fragments were link

SUBMITTER: Neitz H 

PROVIDER: S-EPMC9365081 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature