Taste Receptor Activation in Tracheal Brush Cells by Denatonium Modulates ENaC Channels via Ca²⁺, cAMP and ACh.

ABSTRACT: Mucociliary clearance is a primary defence mechanism of the airways consisting of two components, ciliary beating and transepithelial ion transport (I_SC). Specialised chemosensory cholinergic epithelial cells, named brush cells (BC), are involved in regulating various physiological and immunological processes. However, it remains unclear if BC influence I_SC. In murine tracheae, denatonium, a taste receptor agonist, reduced basal I_SC in a concentration-dependent manner (EC₅₀ 397 µM). The inhibition of bitter taste signalling components with gallein (G_βγ subunits), U73122 (phospholipase C), 2-APB (IP3-receptors) or with TPPO (Trpm5, transient receptor potential-melastatin 5 channel) reduced the denatonium effect. Supportively, the I

SUBMITTER: Hollenhorst MI 

PROVIDER: S-EPMC9367940 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature