Dynamic cybergenetic control of bacterial co-culture composition via optogenetic feedback

ABSTRACT: Communities of microbes play important roles in natural environments and hold great potential for deploying division-of-labor strategies in synthetic biology and bioproduction. However, the difficulty of controlling the composition of microbial consortia over time hinders their optimal use in many applications. Here, we present a fully automated, high-throughput platform that combines real-time measurements and computer-controlled optogenetic modulation of bacterial growth to implement precise and robust compositional control of a two-strain E. coli community. In addition, we develop a general framework for dynamic modeling of synthetic genetic circuits in the physiological context of E. coli and use a host-aware model to determine the optimal control parameters of our closed-loop composit

SUBMITTER: Gutierrez Mena J 

PROVIDER: S-EPMC9381578 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature