Lamprey immune protein triggers the ferroptosis pathway during zebrafish embryonic development.

ABSTRACT:

Background

Previously, a novel lamprey immune protein (LIP) was identified, which plays an important role in immunity and the regulation of growth and development in lampreys. However, the mechanism of how LIP regulates growth and development remains unclear.

Methods

In this study, a zebrafish model of LIP overexpression was established by delivering a transgenic plasmid to the fertilized egg. The biological function of LIP was explored in vivo through phenotypic characterization, comparative transcriptome sequencing, and physiological and biochemical analyses.

Results

LIP caused developmental toxicity in zebrafish, increased embryo mortality and exhibited strong teratogenic, lethal, and developmental inhibitory effects. Comparative transcriptome analysis showed that

SUBMITTER: Du Z 

PROVIDER: S-EPMC9386916 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature