Dataset Information

Draft Genome Sequences of Two Desiccation-Tolerant Strains, Bradyrhizobium japonicum TXVA and TXEA, Isolated from the Root Nodules of Soybean Grown in Texas.

ABSTRACT: Two Bradyrhizobium japonicum strains, TXVA and TXEA, were isolated for their desiccation tolerance and symbiotic performance with soybean as biofertilizers. Their genomes were sequenced and annotated using the Department of Energy Joint Genome Institute annotation pipeline. Sequencing yielded chromosomes of 9,193,770 and 9,339,455 bp for TXVA and TXEA, respectively.

SUBMITTER: Peterson C

PROVIDER: S-EPMC9387295 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Draft Genome Sequences of Two Desiccation-Tolerant Strains, Bradyrhizobium japonicum TXVA and TXEA, Isolated from the Root Nodules of Soybean Grown in Texas.

Peterson Christian C Niraula Sarbjeet S Parks Dylan D Chang Woo-Suk WS

Microbiology resource announcements 20220802 8

Two Bradyrhizobium japonicum strains, TXVA and TXEA, were isolated for their desiccation tolerance and symbiotic performance with soybean as biofertilizers. Their genomes were sequenced and annotated using the Department of Energy Joint Genome Institute annotation pipeline. Sequencing yielded chromosomes of 9,193,770 and 9,339,455 bp for TXVA and TXEA, respectively. ...[more]

PMID: 35916509

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Project description:The growth and persistence of rhizobia and bradyrhizobia in soils are negatively impacted by drought conditions. In this study, we used genome-wide transcriptional analyses to obtain a comprehensive understanding of the response of Bradyrhizobium japonicum to drought. Desiccation of cells resulted in the differential expression of 15 to 20% of the 8,453 [corrected] B. japonicum open reading frames, with considerable differentiation between early (after 4 h) and late (after 24 and 72 h) expressed genes. While 225 genes were universally up-regulated at all three incubation times in response to desiccation, an additional 43 and 403 up-regulated genes were common to the 4/24- and 24/72-h incubation times, respectively. Desiccating conditions resulted in the significant induction (>2.0-fold) of the trehalose-6-phosphate synthetase (otsA), trehalose-6-phosphate phosphatase (otsB), and trehalose synthase (treS) genes, which encode two of the three trehalose synthesis pathways found in B. japonicum. Gene induction was correlated with an elevated intracellular concentration of trehalose and increased activity of trehalose-6-phosphate synthetase, collectively supporting the hypothesis that this disaccharide plays a prominent and important role in promoting desiccation tolerance in B. japonicum. Microarray data also indicated that sigma(54)- and sigma(24)-associated transcriptional regulators and genes encoding isocitrate lyase, oxidative stress responses, the synthesis and transport of exopolysaccharides, heat shock response proteins, enzymes for the modification and repair of nucleic acids, and the synthesis of pili and flagella are also involved in the response of B. japonicum to desiccation. Polyethylene glycol-generated osmotic stress induced significantly fewer genes than those transcriptionally activated by desiccation. However, 67 genes were commonly induced under both conditions. Taken together, these results suggest that B. japonicum directly responds to desiccation by adapting to changes imparted by reduced water activity, such as the synthesis of trehalose and polysaccharides and, secondarily, by the induction of a wide variety of proteins involved in protection of the cell membrane, repair of DNA damage, stability and integrity of proteins, and oxidative stress responses.

Dataset Information

Draft Genome Sequences of Two Desiccation-Tolerant Strains, Bradyrhizobium japonicum TXVA and TXEA, Isolated from the Root Nodules of Soybean Grown in Texas.

Publications

Draft Genome Sequences of Two Desiccation-Tolerant Strains, Bradyrhizobium japonicum TXVA and TXEA, Isolated from the Root Nodules of Soybean Grown in Texas.

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