A Cell Trappable Methyl Rhodol-Based Fluorescent Probe for Hydrogen Sulfide Detection.

ABSTRACT: Hydrogen sulfide is a biologically important molecule and developing chemical tools that enable further investigations into the functions of H₂ S is essential. Fluorescent turn-on H₂ S probes have been developed for use in cellulo and in vivo, but the membrane permeability of these probes can lead to probe leakage and signal attenuation over time. Here we report a cell trappable fluorescent probe for H₂ S, CT-MeRhoAz, which is based on a methylrhodolazide scaffold derivatized with an acetoxymethyl ester group. Prior to ester cleavage, the CT-MeRhoAz probe generates a 2500-fold turn-on response to H₂ S, which is enhanced to a 3000-fold response for the carboxylic acid form of the probe. Additionally, the probe is highly selective for H₂ S over other biologically relevant sulfur, oxygen, and nitrogen-based analytes. Live cell imaging experiments confirmed the biocompatibility of CT-MeRhoAz and also that it is cell trappable, unlike the parent MeRhoAz scaffold.

SUBMITTER: Fosnacht KG 

PROVIDER: S-EPMC9388573 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature