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A biotin-guided hydrogen sulfide fluorescent probe and its application in living cell imaging.

ABSTRACT: Hydrogen sulfide (H₂S), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of H₂S offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent probe, NP-biotin, was developed for H₂S detection and evaluated in normal liver cell (LO2) and liver cancer cell (HepG2) lines. Results reveal that NP-biotin can detect cellular H₂S with high sensitivity and selectivity. Moreover, NP-biotin has been confirmed to possess the ability to target cancer cells under the guidance of the biotin group.

SUBMITTER: Zhang C

PROVIDER: S-EPMC9056979 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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A biotin-guided hydrogen sulfide fluorescent probe and its application in living cell imaging.

Zhang Chen C Zhang Jiewen J Xu Zhiqiang Z Zang Kun K Liu Feng F Yin Jun J Tan Ying Y Jiang Yuyang Y

RSC advances 20200901 59

Hydrogen sulfide (H2S), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of H2S offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent probe, NP-biotin, was developed for H2S detection and evaluated in ...[more]

PMID: 35517112

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