SARS-CoV-2-reactive IFN-γ-producing CD4⁺ and CD8⁺ T cells in blood do not correlate with clinical severity in unvaccinated critically ill COVID-19 patients.

ABSTRACT: We examined the relationship between peripheral blood levels of SARS-CoV-2 S (Spike protein)1/M (Membrane protein)-reactive IFN-γ-producing CD4⁺ and CD8⁺ T cells, serum levels of biomarkers of clinical severity, and mortality in critically ill COVID-19 patients. The potential association between SARS-CoV-2-S-Receptor Binding Domain (RBD)-specific IgG levels in sera and mortality was also investigated. SARS-CoV-2 T cells and anti-RBD IgG levels were monitored in 71 non-consecutive patients (49 male and 22 female; median age, 65 years) by whole-blood flow cytometry and Enzyme-linked immunosorbent assay (ELISA), respectively (326 specimens). SARS-CoV-2 RNA loads in paired tracheal aspirates [TA] (n = 147) were available from 54 patients. Serum levels of interleukin-6, fe

SUBMITTER: Olea B 

PROVIDER: S-EPMC9395536 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature