Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cells.

ABSTRACT: Lymphocytes can heavily influence intestinal health, but resolving intestinal lymphocyte function is challenging as the intestine contains a vastly heterogeneous mixture of cells. Pigs are an advantageous biomedical model, but deeper understanding of intestinal lymphocytes is warranted to improve model utility. Twenty-six cell types were identified in the porcine ileum by single-cell RNA sequencing and further compared with cells in human and murine ileum. Though general consensus of cell subsets across species was revealed, some porcine-specific lymphocyte subsets were identified. Differential tissue dissection and in situ analyses conferred spatial context, revealing similar locations of lymphocyte subsets in Peyer's patches and epithelium in pig-to-human comparisons. Like humans, activa

SUBMITTER: Wiarda JE 

PROVIDER: S-EPMC9396248 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature