Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose.

ABSTRACT:

Background

Production of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-glucose. Therefore, significant effort has been made to develop a one-step vitamin C fermentation process. While, 2-KLG, not vitamin C, is synthesized from nearly all current one-step fermentation processes. Vitamin C is naturally synthesized from glucose in Arabidopsis thaliana via a ten-step reaction pathway that is encoded by ten genes. The main objective of this study was to directly produce vitamin C from D-glucose in Escherichia coli by expression of the genes from the A. thaliana vitamin C biosynthetic pathway.

Results

Therefore, the ten genes of whole

SUBMITTER: Tian YS 

PROVIDER: S-EPMC9396866 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature