Metabolic engineering of Escherichia coli for efficient production of L-5-hydroxytryptophan from glucose

ABSTRACT:

Background

5-hydroxytryptophan (5-HTP), the direct biosynthetic precursor of the neurotransmitter 5-hydroxytryptamine, has been shown to have unique efficacy in the treatment of a variety of disorders, including depression, insomnia, and chronic headaches, and is one of the most commercially valuable amino acid derivatives. However, microbial fermentation for 5-HTP production continues to face many challenges, including low titer/yield and the presence of the intermediate L-tryptophan (L-Trp), owing to the complexity and low activity of heterologous expression in prokaryotes. Therefore, there is a need to construct an efficient microbial cell factory for 5-HTP production.

Results

We describe the systematic modular engineering of wild-type Escherichia coli for the efficien

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC9509612 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature