Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO.
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ABSTRACT:
SUBMITTER: Guo Y
PROVIDER: S-EPMC9405536 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Publications
Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO.
Guo Yue Y Kowalczyk Gabriel J GJ Lee Robin E C REC
STAR protocols 20220818 3
Visualization of mRNA molecules in single cells has revealed their core mechanisms as well as sources of cell-to-cell and spatiotemporal heterogeneity. Here, we describe a protocol to label, visualize, and quantify mRNA molecules by time-lapse imaging with the capability of resolving mRNA molecules over durations of hours to days. We provide links to mRNA-labeling plasmids as well as free software for a semi-automated image analysis pipeline. For complete details on the use and execution of this ...[more]
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