New methods for quantification of amoxicillin and clindamycin in human plasma using HPLC with UV detection.

ABSTRACT:

Objectives

We aimed to develop simple and rapid HPLC methods for determination of amoxicillin and clindamycin in human plasma.

Methods

Plasma samples were pretreated by direct deproteinization with acetonitrile and the analytical separation took place on a reverse phase Poroshell 120 EC-C18 column (2.7 μm, 2.1 × 100 mm) with a gradient of acetonitrile. UV detection at 229 nm for amoxicillin and 204 nm for clindamycin was used for determination of the antibiotics in plasma.

Results

The calibration curves were linear over the concentration ranges of 1-100 mg/L for amoxicillin and 1-15 mg/L for clindamycin with a correlation coefficient of ≥0.98. Intra-assay precisions were all ≤15% and the accuracies were within ±15%. The limit of quantification (LOQ) was found to be 0.

SUBMITTER: Greibe E 

PROVIDER: S-EPMC9410666 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature