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A biomimetic yeast shell vaccine coated with layered double hydroxides induces a robust humoral and cellular immune response against tumors.

ABSTRACT: Enhancing both the humoral and cellular immune response for tumor vaccination remains a challenge. Inspired by natural pathogen structures, we took β-glucan particles derived from a baker's yeast cell shell (YS) as a vaccine carrier and danger signal for dendritic cells (DCs), and coated the YS with catanionic layered double hydroxides (LDH) by electrostatic adsorption to form a biomimetic yeast cell particle (YSL). Our experimental results showed that the YSL vaccine efficiently targeted antigen-presenting cells (APCs) and remarkably enhanced antigen cross-presentation, and strongly improved the activation and maturation of DCs. Moreover, the YSL vaccine elicited an extremely high antibody titer and strong antigen-specific cytotoxic T lymphocyte together with mixed Th1/Th17 cellular immune responses and induced marked prophylactic and therapeutic effects against E.G7-OVA tumors in mouse models. These results suggest that YSL, integrating a yeast shell to mimic natural pathogens and LDH with high antigen-loading capacity and lysosome escape, is a promising tumor vaccine platform for rapid, effective and strong induction of both humoral and cellular immune responses.

SUBMITTER: Liu DQ

PROVIDER: S-EPMC9419453 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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A biomimetic yeast shell vaccine coated with layered double hydroxides induces a robust humoral and cellular immune response against tumors.

Liu Dong-Qun DQ Lu Shuai S Zhang Lun L Zhang Ling-Xiao LX Ji Mei M Liu Xiao-Ge XG Yu Zhuo Z Liu Rui-Tian RT

Nanoscale advances 20200626 8

Enhancing both the humoral and cellular immune response for tumor vaccination remains a challenge. Inspired by natural pathogen structures, we took β-glucan particles derived from a baker's yeast cell shell (YS) as a vaccine carrier and danger signal for dendritic cells (DCs), and coated the YS with catanionic layered double hydroxides (LDH) by electrostatic adsorption to form a biomimetic yeast cell particle (YSL). Our experimental results showed that the YSL vaccine efficiently targeted antige ...[more]

PMID: 36134256

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Project description:Research on two-dimensional materials is one of the most relevant fields in materials science. Layered double hydroxides (LDHs), a versatile class of anionic clays, exhibit great potential in photocatalysis, energy storage and conversion, and environmental applications. However, its implementation in real-life devices requires the development of efficient and reproducible large-scale synthesis processes. Unfortunately, reliable methods that allow for the production of large quantities of two-dimensional LDHs with well-defined morphologies and high crystallinity are very scarce. In this work, we carry out a scale-up of the urea-based CoAl-LDH synthesis method. We thoroughly study the effects of the mass scale-up (25-fold: up to 375 mM) and the volumetric scale-up (20-fold: up to 2 L). For this, we use a combination of several structural (XRD, TGA, and N2 and CO2 isotherms), microscopic (SEM, TEM, and AFM), magnetic (SQUID), and spectroscopic techniques (ATR-FTIR, UV-vis, XPS, ICP-MS, and XANES-EXAFS). In the case of the volumetric scale-up, a reduction of 45% in the lateral dimensions of the crystals (from 3.7 to 2.0 µm) is observed as the reaction volume increases. This fact is related to modified heating processes affecting the alkalinization rates and, concomitantly, the precipitation, even under recrystallization at high temperatures. In contrast, for the tenfold mass scale-up, similar morphological features were observed and assigned to changes in nucleation and growth. However, at higher concentrations, simonkolleite-like Co-based layered hydroxide impurities are formed, indicating a phase competition during the precipitation related to the thermodynamic stability of the growing phases. Overall, this work demonstrates that it is possible to upscale the synthesis of high-quality hexagonal CoAl-LDH in a reproducible manner. It highlights the most critical synthesis aspects that must be controlled and provides various fingerprints to trace the quality of these materials. These results will contribute to bringing the use of these 2D layered materials closer to reality in different applications of interest.

Project description:The development of halogen-free flame retardants as environmentally friendly and renewable materials for heat and fire-resistant applications in the field of electronics is important to ensure safety measures. In this regard, we have proposed a simple and halogen-free strategy for the synthesis of flame retardant LDH-PN materials to decrease the fire hazards of epoxy resin (EP), via a co-precipitation reaction between Mg(NO3)2 and Al(NO3)3 and the subsequent incorporation of different cyclotriphosphazene (PN) carboxylate anions. The cyclotriphosphazene-based di, tetra and hexacarboxylate-intercalated layered double hydroxides are designated as LDH-PN-DC, LDH-PN-TC and LDH-PN-HC, respectively. Furthermore, the intercalation of cyclotriphosphazene carboxylate anions into the LDH layers was confirmed by PXRD, FT-IR, TGA, solid-state 31P NMR, nitrogen adsorption and desorption analysis (BET), HR-SEM and XPS. Evaluation of the flame retardant (vertical burning test and limiting oxygen index) properties was demonstrated by formulating the LDH-PN materials with epoxy resin (EP) in different ratios coated on wood substrates to achieve the desired behaviour of the EP/LDH-PN composites. Structure-property analysis reveals that EP/LDH-PN-TC-20 wt% and EP/LDH-PN-HC-20 wt% achieved a V 0 rating in the UL-94 V test and achieved higher LOI values (27.7 vol% for EP/LDH-PN-TC-20 wt% and 29 vol% for EP/LDH-PN-HC-20 wt%) compared to the epoxy-coated wood substrate (23.2 vol%), whereas EP/LDH-PN-DC failed in the vertical burning test for various weight percentages of LDH-PN-DC from 5 wt% to 20 wt% in the composites, with a lower LOI value of 22.1 vol%. Excellent flame retardancy was observed for EP/LDH-PN-TC and EP/LDH-PN-HC due to the presence of more binding sites of carboxylate anions in the LDH layers and less or no spiro groups in cyclotriphosphazene compared to that in EP/LDH-PN-DC. In addition, the synergistic flame retardant effect of the combination of LDH and cyclotriphosphazene on the epoxy resin composites remains very effective in creating a non-volatile protective film on the surface of the wood substrate to shelter it from air, absorb the heat and increase the ignition time, which prevents the supply of oxygen during the combustion process. The results of this study show that the proposed strategy for designing flame-retardant properties represents the state-of-the-art, competent coating of inorganic materials for the protection and functionalization of wood substrates.

Dataset Information

A biomimetic yeast shell vaccine coated with layered double hydroxides induces a robust humoral and cellular immune response against tumors.

Publications

A biomimetic yeast shell vaccine coated with layered double hydroxides induces a robust humoral and cellular immune response against tumors.

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