The l-rhamnose-dependent regulator RhaS and its target promoters from Escherichia coli expand the genetic toolkit for regulatable gene expression in the acetic acid bacterium Gluconobacter oxydans.

ABSTRACT: For regulatable target gene expression in the acetic acid bacterium (AAB) Gluconobacter oxydans only recently the first plasmids became available. These systems solely enable AraC- and TetR-dependent induction. In this study we showed that the l-rhamnose-dependent regulator RhaS from Escherichia coli and its target promoters P _rhaBAD , P _rhaT , and P _rhaSR could also be used in G. oxydans for regulatable target gene expression. Interestingly, in contrast to the responsiveness in E. coli, in G. oxydans RhaS increased the expression from P _rhaBAD in the absence of l-rhamnose and repressed P _rhaBAD in the presence of l-rhamnose. Inserting an additional RhaS binding site

SUBMITTER: Fricke PM 

PROVIDER: S-EPMC9429829 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature