Dataset Information

SPTSSA Is a Prognostic Marker for Glioblastoma Associated with Tumor-Infiltrating Immune Cells and Oxidative Stress.

ABSTRACT:

Background

SPTSSA encodes the small subunit A of serine palmitoyltransferase. It catalyzes the formation of sphingoid long-chain base backbone of sphingolipids. Its role in glioma prognosis and tumor-infiltrating immune cells remains unclear.

Methods

We analyzed SPTSSA expression and association with clinical prognosis using GEPIA and CGGA database. Then, GSEA was performed to identify relevant biological functions of SPTSSA. The correlations between SPTSSA expression and tumor immune infiltrates were investigated using CIBERSORT and TIMER. Finally, IHC and IF were performed to confirm the value of prognosis and the correlation with immune infiltration.

Results

SPTSSA expression was significantly upregulated in diffuse glioma compared to normal tissues and associated with poor survival in GEPIA and CGGA database. Then, we identified biological processes and signaling pathways associated with SPTSSA expression. The result showed that SPTSSA enriched in the GO term like oxidative stress. Finally, we showed that SPTSSA expression was significantly associated with tumor-infiltrating immune cells and overall survival via IHC.

Conclusion

These findings suggest that SPTSSA expression might be used as a prognostic biomarker for glioma and potential target for novel glioma therapy.

SUBMITTER: Wang Z

PROVIDER: S-EPMC9434331 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

<i>SPTSSA</i> Is a Prognostic Marker for Glioblastoma Associated with Tumor-Infiltrating Immune Cells and Oxidative Stress.

Wang Ziheng Z Ge Xinqi X Shi Jinlong J Lu Bing B Zhang Xiaojin X Huang Jianfei J

Oxidative medicine and cellular longevity 20220824

<h4>Background</h4><i>SPTSSA</i> encodes the small subunit A of serine palmitoyltransferase. It catalyzes the formation of sphingoid long-chain base backbone of sphingolipids. Its role in glioma prognosis and tumor-infiltrating immune cells remains unclear.<h4>Methods</h4>We analyzed <i>SPTSSA</i> expression and association with clinical prognosis using GEPIA and CGGA database. Then, GSEA was performed to identify relevant biological functions of <i>SPTSSA</i>. The correlations between <i>SPTSSA ...[more]

PMID: 36062185

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