Intratumoral heterogeneity of MYC drives medulloblastoma metastasis and angiogenesis.

ABSTRACT:

Background

Intratumoral heterogeneity is crucially involved in metastasis, resistance to therapy, and cancer relapse. Amplifications of the proto-oncogene MYC display notable heterogeneity at the single-cell level and are associated with a particularly dismal prognosis in high-risk medulloblastomas (MBs). The aim of this study was to establish the relevance of interclonal cross-talk between MYC-driven and non-MYC-driven MB cells.

Methods

We used fluorescence in situ hybridization, single-cell transcriptomics, and immunohistochemistry, in vitro isogenic cell models, non-targeted proteomics, mass spectrometry-based metabolite quantification, HUVECs tube formation assay, and orthotopic in vivo experiments to investigate interclonal cross-talk in MB.

Results

We found that

SUBMITTER: Qin N 

PROVIDER: S-EPMC9435486 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature