Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation.

ABSTRACT:

Background

The chicken in ovo model is an attractive system to explore underlying mechanisms of neural and brain development, and it is important to develop effective genetic modification techniques that permit analyses of gene functions in vivo. Although electroporation and viral vector-mediated gene delivery techniques have been used to introduce exogenous DNA into chicken embryonic cells, transducing neurons efficiently and specifically remains challenging.

Methods

In the present study, we performed a comparative study of the ubiquitous CMV promoter and three neuron-specific promoters, chicken Ca2+/calmodulin-dependent kinase (cCaMKII), chicken Nestin (cNestin), and human synapsin I. We explored the possibility of manipulating gene expression in chicken embryonic brain ce

SUBMITTER: Jung KM 

PROVIDER: S-EPMC9440574 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature