Interaction of ARRDC4 With GLUT1 Mediates Metabolic Stress in the Ischemic Heart.

ABSTRACT:

Background

An ancient family of arrestin-fold proteins, termed alpha-arrestins, may have conserved roles in regulating nutrient transporter trafficking and cellular metabolism as adaptor proteins. One alpha-arrestin, TXNIP (thioredoxin-interacting protein), is known to regulate myocardial glucose uptake. However, the in vivo role of the related alpha-arrestin, ARRDC4 (arrestin domain-containing protein 4), is unknown.

Methods

We first tested whether interaction with GLUTs (glucose transporters) is a conserved function of the mammalian alpha-arrestins. To define the in vivo function of ARRDC4, we generated and characterized a novel Arrdc4 knockout (KO) mouse model. We then analyzed the molecular interaction between arrestin domains and the basal GLUT1.

Results

A

SUBMITTER: Nakayama Y 

PROVIDER: S-EPMC9444972 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature