Rapid detection of bovine rotavirus a by isothermal reverse transcription recombinase polymerase amplification assays.

ABSTRACT:

Background

Bovine rotavirus A (BRVA) is considered to be the most common pathogen of severe diarrhea in cattle worldwide, which could lead to the death of newborn calves and cause the significant economic losses to the cattle industry. As a novel isothermal nucleic acid amplification technique, recombinase polymerase amplification (RPA) has been applied widely for the rapid detection of different important pathogens in human and animals.

Results

An RT-RPA assay based on the real time fluorescence monitoring (real-time RT-RPA) and an RT-RPA assay combined with a lateral flow strip (LFS RT-RPA) were successfully developed by targeting the VP6 gene of BRVA. The RT-RPA assays allowed the exponential amplification of the target fragment in 20 min. After incubation of the LFS RT-R

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9453720 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature